This kit is used for the in vitro qualitative detection of human whole blood genomic DNA sample, which can detect the commonly seen 3 kinds of deletion type α-thalassemia gene mutation (–SEA, -α3.7 and -α4.2), 3 kinds of non-deletion type α-thalassemia gene mutation (αCSα, αQSα and αWSα) and 17 kinds of β-thalassemia gene mutation (41-42M, 654M, -28M, 71-72M, 17M, βEM, IVS-I-1M, IVS-I-5M, 27/28M, 43M, -29M, -30M, 31M, -32M, 14-15M, IntM and CAPM)